Journal: Scientific Reports
Article Title: A multicellular brain spheroid model for studying the mechanisms and bioeffects of ultrasound-enhanced drug penetration beyond the blood‒brain barrier
doi: 10.1038/s41598-023-50203-3
Figure Lengend Snippet: Schematic showing generation of the 3D brain spheroid model. Neural precursor cells (NPCs) and astrocytes generated from human induced pluripotent stem cells (hiPSCs) were mixed with human microglia and grown for 2 weeks. Later, human pericytes and human brain microvascular endothelial cells (HBMECs) were added and cultured (~ 2 weeks) until the spheroids were ready for experiments (detailed protocol provided under methods section). The inset shows the average diameter (650 µm) of spheroids used in the study ( n = 100). The images show a typical spheroid. DIC differential interference contrast. Nuclei are counterstained with DAPI (blue). Scale bar = 100 µm.
Article Snippet: The neural precursor cells (NPCs) derived from human induced pluripotent stem cell (hiPSC) line SC0000020 (subclone SF) (generated at RUCDR Infinite Biologics, Piscataway, NJ, USA) were maintained in STEMdiff Neural Progenitor Medium (05833, STEMCELL Technologies, Vancouver, BC, Canada).
Techniques: Generated, Cell Culture